MHC Class II DQ/DR antibody (FITC)

Catalog No. ABIN625970

The Mouse Monoclonal anti- antibody has been validated for FACS, IHC, IHC (fro), IP and IHC (p). It is suitable to detect in samples from Sheep, Cow, Goat and Human.

Quick Overview for MHC Class II DQ/DR antibody (FITC) (ABIN625970)

Target: MHC Class II DQ/DR

Reactivity: Sheep, Cow, Goat, Human

Host: Mouse

Clonality: Monoclonal

Conjugate: FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 49-1

Product Details

Brand: IHC-plus™

Specificity: Recognizes a polymorphic epitope on ovine MHC class II DQ and DR molecules. In recent work, this clone was found to recognize ovine MHC class II transfectants DQ - T28.1. DR - T8.1. DR - T31.3, but not DQ - T26.2 (Ballingall, K. et al.1995) The expression of ovine MHC class II molecules is restricted to B cells, activated T cells, monocytes, macrophages, dendritic cells and some endothelial and epithelial cells.

Purification: Affinity purified

Immunogen:

Type of Immunogen: Native protein

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo (1:10), IHC, IHC-Fr, IHC-P (1:50), IP

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. Flow Cytometry: Use 10 μL of the suggested working dilution to label 1 x 10^6 cells in 100 μL. Method sheets are available on request.

Comment:

Target Species of Antibody: Sheep

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide, 1 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details

Target: MHC Class II DQ/DR

Alternative Name: MHC Class II DQ DR Polymorphic